Topics of emerging importance:
- Viable but non-cultivable (VNC) microbes
- Interspecies and intraspecies communication among microorganisms using physical signals
Viable but non-cultivable (VNC) bacteria were estimated by difference between viable and VC counts. Isolates were clustered by phenotype.
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ReplyDeleteVNBC cells that are metabolically or physiologically active but cannot be cultured on specific media.Most of the bacteria that enter VBNC state are gram-negative species belonging to the gamma subclass of the proteobacteria branch.
ReplyDeleteVBNC cells exhibit active metabolism in the form of respiration or fermentation, incorporate radioactive substrates, and have active protein synthesis but cannot be cultured or grown on conventional laboratory media.
VNBC lactic acid bacterium use in fermentation process:
In this procedure Quantitative Approach is use to determine the contribution of VNBC state in malolactic fermentation.where use of Different sizes of viable-but-nonculturable cell subpopulations of a lactic acid bacterium strain were induced by adding increasing amounts of SO2.
cider malolactic fermentation (MLF) was selected as a model system to clarify the role played by VBNC cells in bioprocesses .MLF was carried out under different SO2 concentrations for inducing VBNC states.
there results suggest:
VBNC cells conducting MLF showed a state of reduced metabolic activity. The specific malate uptake rate of the VBNC subpopulation was approximately 50% of that found for the viable population in all cases tested, irrespective of medium composition and SO2 concentration.
VBNC cells became the majority of the total population in all cases (70 to 90%). These results help to clarify, in quantitative terms, the real contribution of the VBNC subpopulation to fermentation processes. This outcome could be useful for bioprocess control and optimization on an industrial scale. ..
Details of of these methods is given in review article:
Quantitative Approach to Determining the Contribution of Viable-but-Nonculturable Subpopulations to Malolactic Fermentation Processes.
follow the link given below
http://aem.asm.org/content/75/9/2977.full
Viable but nonculturable (VBNC) bacteria refers to bacteria that are in a state of very low metabolic activity and do not divide, but are alive and have the ability to become culturable once resuscitated.
ReplyDeleteBacteria in a VBNC state cannot grow on standard growth media, though flow cytometry can measure the viability of the bacteria.Bacteria can enter the VBNC state as a response to stress, due to adverse nutrient, temperature, osmotic, oxygen, and light conditions.The cells that are in the VBNC state are morphologically smaller, and demonstrate reduced nutrient transport, rate of respiration, and synthesis of macromolecules.Sometimes, VBNC bacteria can remain in that state for over a year.It has been shown that numerous pathogens and non-pathogens can enter the VBNC state, and therefore it has significant implications in pathogenesis, bioremediation, and other branches of microbiology.
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